Monitor and measure the expression of pluripotency markers
With SBI’s line of pGreenFire Pluripotency Response Reporter Vectors, you can monitor and measure the expression of pluripotency markers in live cells in real time. These vectors leverage our reliable lentivector technology and save you time—the pre-built reporters come as ready-to-package lentivector plasmids, with some reporters also available as ready-to-transduce, pre-packaged virus. All of our Response Reporters deliver great performance in both human and mouse cells.
The Sox2 SRR2 pGreenFire (EF1α-puro) Response Reporter uses the Sox2-responsive SRR2 enhancer with a mouse CMV promoter to co-express GFP and luciferase. Co-expression is mediated by a T2 element. The minimal CMV promoter alone delivers negligible expression, but when downstream of SSR2 enhancer elements, drives expression of dscGFP and luciferase under conditions where Sox2 is also expressed. The result is the ability to quantitatively measure Sox2 expression by fluorescence and luciferase activity.
The vector also includes a constitutively-expressed puromycin marker (EF1α promoter) to simplify cell line construction. Or choose a constitutively expressed neomycin marker (Cat.# SR20071-PA-N), or no marker at all (Cat.# SR20071-PA-1).
- Create stable cell lines for quantitation of Oct4 expression
- Track differentiation in live cells in real time
Please note that these vectors only function properly when transduced. Transfection keeps the constitutive RSV promoter intact, leading to nonspecific expression of the reporter genes.
Choose the right vector to monitor pluripotency markers (Pluripotency Reporters) or to measure the activity of pluripotency makers (Pluripotency Response Reporters)
Pluripotency response reporters
| SBI’s Pluripotency Response Reporters work for both human and mouse stem cells | |||
|---|---|---|---|
| Catalog # | Promoter/Enhancer elements | Reporter | Selection cassette |
| SR20070-PA/VA-1 | Oct4 CR4 | pGreenFire (GFP-Luc) | None |
| SR20070-PA-N | Oct4 CR4 | pGreenFire (GFP-Luc) | EF1α-Neo |
| SR20070-PA-P | Oct4 CR4 | pGreenFire (GFP-Luc) | EF1α-Puro |
| SR20071-PA/VA-1 | Sox2 SRR2 | pGreenFire (GFP-Luc) | None |
| SR20071-PA-N | Sox2 SRR2 | pGreenFire (GFP-Luc) | EF1α-Neo |
| SR20071-PA/VA-P | Sox2 SRR2 | pGreenFire (GFP-Luc) | EF1α-Puro |
| Catalog # | Species | Promoter/enhancer element | Reporter |
|---|---|---|---|
| SR10033PA/VA-1 | Human | Oct-4 | pGreenZeo |
| SR10043PA/VA-1 | Human | Oct-4 | pRedZeo |
| SR10053PA/VA-1 | Human | Oct-4 | pRedTK |
| SR10029PA/VA-1 | Mouse | Oct-4 | pGreenZeo |
| SR10045PA/VA-1 | Mouse | Oct-4 | pRedZeo |
| SR10054PA/VA-1 | Mouse | Oct-4 | pRedTK |
| SR10030PA/VA-1 | Human | Nanog | pGreenZeo |
| SR10042PA/VA-1 | Human | Nanog | pRedZeo |
| SR10055PA/VA-1 | Human | Nanog | pRedTK |
| SR10031PA/VA-1 | Mouse | Nanog | pGreenZeo |
| SR10044PA/VA-1 | Mouse | Nanog | pRedZeo |
| SR10056PA/VA-1 | Mouse | Nanog | pRedTK |
